Midsci's Feburary backpack winner!

Congratulations to Midsci St Louis's February backpack winner is Bob Mikesell from the Cross lab at Ophthalmology Wash U! Woohoo! Bob has been a long time TPP user, thank you for your loyality to TPP!

HIV CURE

This week some very exciting news is hitting the presses on a possible cure for HIV: http://www.huffingtonpost.com/2010/12/14/hiv-cure-berlin-patient_n_796521.html We are so very proud of the achievements of these researchers and our hopes that this is a real breakthrough are of a deep heartfelt nature.

Also, kudos to all researchers that study HIV and/or work with Stem Cells. You all played a part; somewhere, in some way, of progressing basic sciences to huge achievements.

Notice the picture from the lab: TPP 50ml Tubes, 75cm2 Flasks, and our Cell Scrapers were the products of choice. If TPP played any small part in that we are humbled and honored.

Now what else can be done?

TPP Cited in Paper

Thank you to Simon Kaja, PhD for citing TPP and sending us the link.

Dr. Kaja is the Associate Director Preclinical Research Vision Research Center; Assistant Professor in the Departments of Ophthalmology and Basic Medical Science, University of Missouri - Kansas City. You can learn more about Dr. Kaja work at www.simonkaja.com.

Here is a link to his latest paper: Novel mechanism: oxidative stress selectively increases type-2 IP3 receptors in neuronal cells. http://bit.ly/9zAiZc

If you want 1000's more to see your paper, send us a link and we will post it here, at FB and Twitter!

How much does it cost you for a bad 50ml centrifuge tube?

Freezer Clean-Out: How much do you throw away because it was mislabeled or not labeled? TPP tubes come with a chemical and liquid resistant label on the cap.

Tube Breakage/Tube Leakage: How much does a broken or leaking tube cost you? If it breaks in the centrifuge and it is radioactive, it is 3 days and a lot of hassle. If it has 25ml of FBS in it, then that $0.20 tube cost you $10 in loss. 100% of all TPP tube lots are tested to meet specs: 9400xg (PP), to -180oC and up to 121oC.

Tired of Tubes Filled w/Dark Liquid & Have Dark Printed Graduations? All of TPP Tubes have white graduations and white writing surfaces.

Why Do Tubes Only Have Graduations to 5ml? TPP 50ml has graduations to 0.5ml and the 15ml has them to 0.1ml.

Buy some today and try them for yourself!

NEW 600ml Bioreactor Tube

Introducing the new Bioreactor 600! The TPP Bioreactor family has grown from the 50ml tube to now including a 600ml tube. Ideal for larger scale suspensions, parallel experiments, bioreacting, and more, this tube adds versatility, volume, and convenience to any lab.

With a maximum working volume of 400ml and a 0.2um filter cap this tube fits a wide variety of applications. Whether shaking or centrifuging the tube also fits most standard rotors or tube racks for 500ml tubes.

Want to learn more about this product? Contact us at 800-544-9909 or tech@tpp-us.com or watch this video about the Bioreactor Tubes: http://www.youtube.com/watch?v=AdifEb0nezA.

Helpful hints for aseptic techniques

Want better aseptic techniques? For more information click here: http://www.midsci.com/images/Blog/HelpfulHintsBetterAsepticTechnique.pdf.

10cm Tiny Flasks

Have you ever wished you could just grow your adherent cells in a tube, so you could save some time and an extra step in the cell culture hood? TPP 10cm Tiny Flasks solve that problem! The tube is approximately the same size as a 50ml tube, though it has 2 flat sides-one 10cm2 growth area and one flat viewing window for microscopy. The tube will also not fall through a microscope stage like a 35mm dish can. These tubes also have a plastic resting mount to keep the tubes in place in the incubator and to give the tube the optimal angle for the growth surface. The cleverly designed tear-drop shape of the growth surface can be exactly worked with cell scraper as well. See this tube in action at: http://tpp-us.com/docs/tpp-tissue-culture-flask-tube.html.

AlphaPette Blowout!

Pipettors specifically for the tissue culture hood are a necessity for any lab worried about contamination. And now is the perfect time to stock up on them-Midsci is having a huge sale! The AlphaPettes are fully autoclavable, which means you can keep your pipettes sterile for working in the hood. The continuous adjustment with either the plunger button or the thumbwheel means your dispensing volumes are always accurate. The lifetime warranty doesn't hurt either! They also accept universal tips, so you can use the filter tips of your choice. Sale prices range from $139-149, call or email Midsci for more information. You can find the pipettors at Midsci's website: http://thelabshark.com/cat/prodprice2_Detail.cfm?ID=942.

Do you have AlphaPettes? What do you like about them? What would you like to see from future generations of AlphaPettes? Leave your comments!

TPP 1L Filtermax Bottle Filter Preview!

Introducing, due to popular request, the new TPP 1000ml Filtermax bottle filters and bottle-top filters. These new bottle filters will offer the same great features as the other bottle filters TPP offers, only in a larger size. The square shape of the bottles and filters means less space taken up in a refrigerator, on a shelf, or stored in boxes. This shape also helps in handling the bottles, as well as giving the bottle a sturdier footprint, which makes the bottle more difficult to tip over while filtering. The large surface area of the PES membrane offers extremely low protein binding and excellent chemical resistance.

The prototypes for the 1L bottle filters are getting great reviews in the lab and should be available to everyone late fall. Until then, you can see the full line of bottle filters through Midsci at http://thelabshark.com/cat/prodprice2_Detail.cfm?ID=2060 and http://thelabshark.com/cat/prodprice2_Detail.cfm?ID=2220.

Using the TPP Easy Read Device

The "Easy Read" PCV (Packed Cell Volume) Tubes provide a quicker, less tedious alternative to counting cells than using a microscope and hemacytometer. Users simply load a sample of cell suspension into the PCV Tube, spin for 1 minute in a microcentrifuge to pellet the cells into the capillary at the bottom of the tube, and then accurately determine the cell pellet volume with the "Easy Read" measuring device. The cell pellet volume can then be utilized in conjunction with pre-determined conversion factors to quickly calculate cell number, protein production, or metabolic activity.

The Easy Read device is a blue metal ruler that measures microliter amounts. The front part of this ruler is flat and allows the user to measure microliter amounts to 0.025ul. Behind the ruler on the front is a metal slant on the back going down toward the higher measurements. The gray metal guide includes both a magnifying glass as well as the tube holder. The bottom of the tube is placed into the holder and rests against the slant on the back of the device. The tube then slides up and down that slant as you move the gray metal piece across the ruler. Once the top of your pellet is flush with the ruler, you can read the measurement through the magnifying glass.

Using the Easy Read device can give you more accurate results over the capillary graduations on the regular PCV tubes. The cost savings in buying the Easy Read device and using the non graduated tubes adds up quickly after multiple samples. http://tpp-us.com/docs/PCV_Easy_Read_Cell_Counting_System.html

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